Biochemistry and Molecular Biophysics

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Logo for the Dept of Biochemistry and Molecular Biophysics at Washington University in St. Louis, MO
Logo for the Dept of Biochemistry and Molecular Biophysics at Washington University in St. Louis, MO

Cori Lecture

Faculty News

Student Awards

Thesis Examinations


Chili Cookoff! And the winner is ...

Welcome to New BMB Arrivals

Research News
2017 Carl and Gerty Cori Lecture

Dr. Taekjip Ha, from Johns Hopkins University, presented this year’s Carl and Gerty Cori Lecture on February 28, 2017 in Connor Auditorium, Farrell Learning and Teaching Center. Dr. Ha is the Bloomberg Distinguished Professor of Biophysics and Biophysical Chemistry with the Johns Hopkins University and an investigator of the Howard Hughes Medical Institute. (For more information, click here)

Faculty News
Dr. Galletto Receives Tenure

Congratulations to Dr. Roberto Galletto, whose promotion with tenure was officially approved by the Board of Trustees on March 3, 2017.
2016 Mentor of the Year Award
Dr. Michael Greenberg, Assistant Professor of Biochemistry and Molecular Biophysics, was chosen to receive the Undergraduate Research Mentor of the Year Award.

Each fall after the completion of funded summer research projects, the Office of Undergraduate Research ask their students to nominate mentors for outstanding contributions to their success as undergraduate researchers.
Dr. Greenberg’s summer student, Paige Cloonan, nominated Michael. She described his caring and diligent attention to her development as a researcher.

Dr. Greenberg received the award at the Fall Undergraduate Research Symposium on Sat. Oct. 29th at noon after a keynote address.

Congratulations, Dr. Greenberg!

2016 Distinguished Service Teaching Award

Congratulations to Dr. Linda Pike, who received the Distinguished Service Teaching Award from the medical students in recognition of her teaching in the Molecular Foundations of Medicine course.
Novel approach shows promise against UTIs

A story about Jim Janetka’s research on antibiotic-sparing therapeutics, prompted by his new paper in J Med Chem, has just been published in The Record. His research group has developed small molecules that prevent bacteria from sticking to the bladder wall, halting development of urinary tract infections in mice.

The technology is the basis of his startup company, Fimbrion Therapeutics, who have recently entered into a collaboration with GlaxoSmithKline to further develop the lead compounds for human clinical trials.

You can view the story here (

Tioma Therapeurics
Contributed by Bill Frazier
Ten years ago I was on the phone with Andy Hoyne, a local attorney willing to help start-ups with no money, trying to think up a name for a new company that would develop antibodies to CD47 that could be used to enhance nitric oxide signaling to treat any number of potential diseases characterized by ischemia-reperfusion injury (IRI).  That day we founded Vasculox, Inc, a company with no money, no facilities, no products and no employees.  The next year, I reconnected with Dr. Pamela Manning, a former post doc in the Department of Pharmacology at WUMS. Having spent a few decades at Pfizer, much of that time working on nitric oxide,  she was looking for a new opportunity.  Pam became interested in Vasculox and signed on as a cofounder.  Over the next few years , working at our kitchen tables, we wrote business plans (not really knowing what one looked like), applications for licensing a patent (not knowing if we really needed the license) and STTR/SBIR grants, several of which were funded (totalling over $4 million dollars) allowing us to start developing our products, humanized anti-CD47 antibodies.  Biogenerator also stepped in early and provided much needed seed funding and access to their new facility at Cortex in which to grow the company. With a lot of help from other WUMS colleagues including Dr. Will Chapman, Head of Abdominal Transplant, and other colleagues at NIH, we focussed on using the antibodies to treat transplanted organs to reduce IRI, a major cause of transplant failure.  Meanwhile, in my lab, we identified certain anti-CD47 antibodies that have the unique property of killing cancer cells but not normal cells.  This, along with the fact that cancer therapy is a much bigger market than organ tranplant, led our new management, also ex-Pfizer, to focus Vasculox’ efforts on cancer therapeutics.  Preclinical data developed at Vasculox, along with data from a number of academic groups at other universities, persuaded Rivervest, a local VC,  to lead a syndicate of four VC groups in a series A funding round of $86 million.  Along with the funding has come a new team of executives and board members with decades of experience and a new name, Tioma Therapeutics, Inc.  Our kitchen table company is now focused on beginning clinical trials in the near future  to understand the potential of anti-CD47 antibodies in treating a wide range of cancers.
For more information about Tioma Therapeurics, visit
Student Awards and Fellowships
2017 DeGutis Prize in Chemical
Biology/Medicinal Chemistry

On May 5th, 2017, Ms. Whitney Grither was awarded the 2017 Ceil M. DeGutis Prize in Chemical Biology/Medicinal Chemistry. Whitney presented her research entitled: Selective Small Molecule Inhibition of Discoidin Domain Receptor 2. Whitney graduated this Spring from the Biochemistry graduate program. She finished her PhD work in the laboratory of Dr. Greg Longmore with the express purpose of identifying novel modes of inhibiting an underappreciated collagen receptor, DDR2, which they had just shown was critical for breast cancer metastasis in experimental mouse models and humans (Nature Cell Biology, 2013). (For more information, click here.)

Congratulations to Joseph Stodola for being named the Olin Biomedical Science Fellow

Dr. Stodola obtained his PhD degree in May 2016 in Peter Burgers’ lab. He is currently working at Sigma-Aldrich.

These Fellowships were created by a generous gift from the Olin Foundation. The Olin Fellowships are presented to Ph.D. and M.D./Ph.D. students conducting research in the biomedical sciences in any Washington University graduate program who have made significant contributions, and demonstrated the potential to become outstanding research scientists.
Congratulations to Margery Gang for receiving the 2016 David F. Silbert Summer Fellowship Award

During the 2016 summer, Margery Gang, a second-year medical student, was the recipient of the David F. Silbert Summer Fellowship award.

Supported by this award, Ms. Gang performed research in the laboratories of Drs. Matthew Christopher and Timothy Ley in the Division of Oncology of the Department of Medicine. Her project was entitled “WT1 loss-of-function mutations in Acute Myeloid Leukemia”. (For more information, click here.)

Congratulations to Drake Jensen for being named the 2016 Gary K. Ackers Fellow

Mr. Jensen received his B.A. in Chemistry and B.S. in Biology from Southern Illinois University Edwardsville in 2013. During this time, he began pursuing research in equilibrium and kinetic studies of Calmodulin target recognition. After graduation he continued research in the same lab and graduated in 2015 with a M.S. in Chemistry. He joined the Division of Biological and Biomedical Sciences at Washington University the same year as part of the Computational & Molecular Biophysics program.  (For more information, click here.)
Congratulations to Robb Welty for being names the 2016 Elliot Elson Fellow

Mr. Welty received his B.S., double majoring in chemistry and biochemistry, from the University of Minnesota Duluth. During that time, he began doing research studying the effects of molecular crowding on diffusion using fluorescence correlation spectroscopy (FCS). After graduation he continued working in the same laboratory and in 2013 graduated with a M.S. in chemistry. He joined the Division of Biological and Biomedical Sciences at Washington University the same year as part of the Computational & Molecular Biophysics program.  (For more information, click here.)

Congratulations to Sarem Hailemariam for being awarded the 2016 MilliporeSigma Fellowship in memory of Dr. Gerty Cori

Ms. Hailemariam received her undergraduate degree in Biology from Shaw University in Raleigh, North Carolina. She joined the Division of Biological and Biomedical Sciences at Washington University in St. Louis in 2012 as part of the Molecular Cell Biology program. Ms. Hailemariam has always had scientific interests in mechanisms employed by different organisms to maintain genome integrity. Prior to joining DBBS, Sarem worked on the mammalian DNA replication origin-licensing factor, Cdt1.   (For more information, click here.)

Congratulations to Lindsey Steinberg for being awarded the David F. Silbert Outstanding Teaching Assistant Award

The 2015-2016 David F. Silbert Outstanding Teaching Assistant Award goes to Ms. Lindsey Steinberg for her work in the Microbes & Pathogenesis course. Ms. Steinberg was nominated by Dr. Henry Huang. Ms. Steinberg exemplified the spirit of this award through her dedication and lasting contribution to the course, the course master, and the students in the class.  (For more information, click here.)

Thesis Examinations
Jayan Rammohan defended his PhD thesis entitled "Regulatory mechanisms of mycobacterial transcription initiation" on September 30, 2016. Jay was in the Computational and Molecular Biophysics program and completed his thesis in the laboratory of Dr. Eric Galburt. 
Alex Holehouse defended his PhD thesis entitled "Sequence Determinants of the Individual and Collective Behaviour of Intrinsically Disordered Proteins" on May 24, 2017.  Alex was in the Computational and Molecular Biophysics program and completed his thesis work in the laboratory of Dr. Rohit Pappu. 
Shannon Ohlemacher defended her PhD thesis entitled "Novel Insights into Yersiniabactin Biosynthesis in Uropathogenic Escherichia coli" on May 12, 2017.  Shannon was in the Biochemistry program and completed her thesis work in the laboratory of Dr. Jeffrey Henderson. 
Shankar Parajuli defended his PhD thesis entitled "Human RNaseH1 limits R loops and facilitates DNA replication" on May 15, 2017.  Shankar was in the Biochemistry program and completed his thesis work in the laboratory of Dr. Sheila Stewart. 
Kwabena (Koby) Sarpong defended his PhD thesis entitled "Biophysical Studies of the Intracellular Domains of the EGFR family of Receptor Tyrosine Kinases" on March 21, 2017.  Koby was in the Biochemistry program and completed his thesis work in the laboratory of Dr. Ron Bose. 
Stephanie Scherer defended her PhD thesis entitled "Novel regulation of neuronal Gα13 signaling by R7-RGS complexes" on July 15, 2016.  Stephanie was in the Biochemistry program and completed her thesis work in the laboratory of Dr. Kendall Blumer. 
2016 Retreats
2016 SLU and WU Joint Retreat

The SLU/WU Biochemistry Joint Retreat was held on October 7, 2016 at the Danforth Plant Science Center. The day featured various talks from students, postdocs and faculty from the Biochemistry departments at both institutions.

Click the following links to view more photos or a slideshow of the photos taken at the event.

2016 Biochemistry & Molecular Biophysics Graduate Program Retreat

The BCM/CMBP Retreat was held on October 21-22, 2016 at the Cedar Creek Conference Center.  Various student talks were presented during the day by senior students in the program.  The guest speakers for the evening session were presented by two WU faculty members.  The first talk was presented by Dr. Greg Bowman, Assistant Professor in Biochemistry & Molecular Biophysics, whose talk was entitled "Controlling proteins' funstions by exploiting their uncharted conformations". The second talk was presented by Dr. Sergej Djuranovic, Assistant Professor in Cell Biology, whose talk was entitled "Mechanisms of translational control during translation elongation".

Talk Winner:
Alex Holeshouse

Chili Cookoff.  And the winner is ....
On April 21st, 2017, we held our 3rd Annual Chili Cook-off. This year included a few non-chili options such as vegan and Chinese selections. The best chili was awarded to The Burgers Lab. Congratulations!  The Burgers lab won a free lunch for the entire lab.

Click on the following to view more photos or a slideshow of the photos taken at the event.
BMB Department Welcomes...
Drake Jensen-Graduate student joined Dr. Eric Galburt's lab.
Melanie Sparks-Graduate student joined Drs. Peter Burgers and Roberto Galletto's labs.
Andrea Soranno-Assistant Professor.
Rui Zhang-Assistant Professor.
Becky Owens-Administrative Coordinator in the Administrative office.
Rui Ma-Postdoctoral Research Associate in Dr. Rui Zhang's lab.
Elson Fellowship Endowment Fund

The colleagues, friends and trainees of Dr. Elliot L. Elson have expressed a wish to establish an endowed education and training fund to honor him and his extraordinary impact on both the Washington University School of Medicine and molecular biophysics. His teaching and research contributions have been far-reaching and numerous.

The Elliot L. Elson Education Training Endowment will be used to support opportunities for students and the very best post-doctoral trainees in the Department of Biochemistry and Molecular Biophysics.

If you would like to help contribute to this endowment fund, please click here.
Research Awards
Gregory Bowman, PhD, Assistant Professor of Biochemistry and Molecular Biophysics received a two year subcontract from the National Institute of Allergy and Infectious Diseases for his project entitled “Therapeutics Targeting Filoviral Interferon-Antagonist and Replication Functions”.  This project is a subcontract with Drs. Gaya Amarsinghe (Wash U) and Christopher Basler (Georgia State University).

Eric Galburt, PhD, Assistant Professor, Department of Biochemistry and Molecular Biophysics, along with Christina Stallings, PhD, Assistant Professor, Department of Molecular Microbiology, received an equipment supplement grant award from the National Institute of General Medical Sciences for their MPI research entitled “Investigating Novel Mechanisms of Transcription Initiation Regulation in Mycobacteria”.
Eric Galburt, PhD, Assistant Professor, Department of Biochemistry and Molecular Biophysics, received a new four year grant award from the National Institute of General Medical Sciences for his research entitled “Mechanisms of Eukaryotic Transcription Initiation”.

Roberto Galletto, PhD, Associate Professor of biochemistry and molecular biophysics received a four year grant award renewal from the National Institute of General Medical Sciences for his research entitled “Helicase Activity and its Role in Telomere and Telomerase Regulation”.

Michael Greenberg, PhD, Assistant Professor of Biochemistry and Molecular Biophysics has been awarded a CDI Micro-Grant from the Washington University Center for Cellular Imaging to use high-resolution imaging techniques to study familial cardiomyopathies in stem cell-derived cardiomyocytes.
Michael Greenberg, PhD, Assistant Professor of Biochemistry and Molecular Biophysics received an award from the CDI hPSC Core Pilot Grant Program to develop models of familial cardiomyopathies using stem cells.

Jim Janetka, PhD, Associate Professor of Biochemistry and Molecular Biophysics and Chemistry Adjunct, along with Scott Hultgren, PhD, Helen L. Stoever Professor of Molecular Microbiology received and a four year grant award from the National Institute of Diabetes and Digestive and Kidney Diseases for their research entitled “Small Molecule Bacterial Lectin Antagonists for UTI Treatment and Prevention”.

Jim Janetka, PhD, Associate Professor, Department of Biochemistry and Molecular Biophysics, received a new two year grant award from the Alvin J. Siteman Cancer Research Fund for his research entitled “Inhibitors of growth factor activation as new adjunct chemotherapy for cancer”.

Timothy M. Lohman, PhD, professor of biochemistry and molecular biophysics received an equipment supplement grant award from the National Institute of General Medical Sciences for his research entitled “SSB Protein/DNA Interactions”.

Timothy M. Lohman, PhD, professor of biochemistry and molecular biophysics received a four year grant award from the National Institute of General Medical Sciences for his research entitled “Helicase Catalyzed DNA Unwinding”.

Upcoming Events for the Fall:
(check BMB website in September for seminar titles)

September 12, 2018
BMB Seminar
Dr. Xuhui Huang
The Hong Kong University of Science and Technology
"From Molecular Dynamics to Genomic Biology: Constructing Kinetic Network Models to Elucidate Transcriptional Fidelity of RNA Polymerase II"

September 19, 2017
BMB Seminar
Dr. Kristen Lynch
University of Pennsylvania
"Getting sick of splicing: Alternative Splicing and the Human Immune System"

October 3, 2017
BMB Seminar
Dr. Vince Voelz
Temple University
"Molecular simulation and Markov State Model approaches for folding, binding and design"

October 10, 2017
BMB Seminar
Dr. Mario Feldman
Washington University in Saint Louis
"Biogenesis of outer membrane vesicles in pathogenic and commensal bacteria"

October 17, 2017
BMB Seminar
Dr. Whitney Yin
University of Texas Medical Branch at Galveston
"A United Mechanism for Mitochondrial DNA repair"

October 26, 2017
BMB Seminar
Dr. Greg Bowman
Washington University in Saint Louis
"Understanding and exploiting allosteric networks"

October 31, 2017
BMB Seminar
Dr. Manu Platt
Georgia Tech
"Quantitative Dissection of Proteolytic Networks Governing Tissue Remodeling in Health and Disease"

November 7, 2017
BMB Seminar
Dr. Julie Biteen
University of Michigan
"Measuring Biochemistry with Biophysics: Watching How Single Molecules Work Together Inside Living Bacteria Cells"

November 14, 2017
BMB Seminar
Dr. Jeff Gelles
Brandeis University
"Non-equilibrium regulation of DNA transcription"

November 28, 2017
BMB Seminar
Dr. Courtney Aldrich
University of Minnesota
"Rationale Design of Antibiotics from First Principles"

December 5, 2017
BMB Seminar
Dr. Yann Chemla
University of Illinois at Urbana-Champaign
"Molecular switch control of nucleic-acid processing machines"

December 12, 2017
BMB Seminar
Dr. Jacqueline Elise Payton
Washington University in Saint Louis
"Transcriptional Deregulation in Lymphoma: the Role of Enhancers and LncRNAs"

Latest Publications:

Yiling Mi, Marcy Coonce, Dorothy Fiete, Lindsay Steirer, Gabriela Dveksler, R. Reid Townsend, and Jacques U. Baenziger. Functional Consequences of Mannose and Asialoglycoprotein Receptor Ablation. J Biol Chem. 2016 Sep 2;291(36):18700-17. doi: 10.1074/jbc.M116.738948. Epub 2016 Jul 12.

Singh S, Bowman GR. Quantifying allosteric communication via both concerted structural changes and conformational disorder with CARDS. J Chem Theory Comput. 2017 Apr 11;13(4):1509-1517. doi: 10.1021/acs.jctc.6b01181. Epub 2017 Mar 22
Sun, X., Laroche, G., Wang, X., Ågren, H., Bowman, G.R., Giguère, P.M., Tu, Y. Propagation of the Allosteric Modulation Induced by Sodium in the δ-Opioid Receptor. Chemistry. 2017 Apr 3;23(19):4615-4624. doi: 10.1002/chem.201605575. Epub 2017 Mar 20.

Shen G, Cui W, Zhang H, Zhou F, Huang W, Liu Q, Yang Y, Li S, Bowman GR, Sadler JE, Gross ML, Li W. Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer. Nat Struct Mol Biol. 2017 Jan;24(1):69-76. doi: 10.1038/nsmb.3333. Epub 2016 Dec 5.

Bowman GR. Accurately modeling nanosecond protein dynamics requires at least microseconds of simulation. J Comput Chem. 2016 Mar 5;37(6):558-66. doi: 10.1002/jcc.23973. Epub 2015 Jun 16
Hart KM, Ho CM, Dutta S, Gross ML, Bowman GR. Modelling proteins' hidden conformations to predict antibiotic resistance. Nat Commun. 2016 Oct 6;7:12965. doi: 10.1038/ncomms12965. Epub link

Hart KM, Moeder KE, Ho CMW, Zimmerman MI, Frederick TE, Bowman GR. Designing small molecules to target cryptic pockets yields both positive and negative allosteric modulators. PLoS One. 2017 Jun 1;12(6):e0178678. doi: 10.1371/journal.pone.0178678. eCollection 2017.

Brosey CA, Ho C, Long WZ, Singh S, Burnett K, Hura GL, Nix JC, Bowman GR, Ellenberger T, Tainer JA. Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor. Structure. 2016 Dec 6;24(12):2067-2079. doi: 10.1016/j.str.2016.09.012. Epub 2016 Nov 3

Pascolutti R, Sun X, Kao J, Maute RL, Ring AM, Bowman GR, Kruse AC., Structure and Dynamics of PD-L1 and an Ultra-High-Affinity PD-1 Receptor Mutant.Structure. Structure. 2016 Oct 4;24(10):1719-1728. doi: 10.1016/j.str.2016.06.026. Epub 2016 Sep 8.

Zimmerman MI, Bowman GR., FAST Conformational Searches by Balancing Exploration/Exploitation Trade-Offs. J Chem Theory Comput. 2015 Dec 8;11(12):5747-57. doi: 10.1021/acs.jctc.5b00737. Epub 2015 Nov 20.

Burgers PM, Kunkel TA. Eukaryotic DNA Replication Fork. Annu Rev Biochem. 2017 Mar 1. doi: 10.1146/annurev-biochem-061516-044709. [Epub ahead of print]

Kochenova OV, Bezalel-Buch R, Tran P, Makarova AV, Chabes A, Burgers PM, Shcherbakova PV. Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations. Nucleic Acids Res. 2017 Feb 17;45(3):1200-1218. Epub Link

Sawicka M, Wanrooij PH, Darbari VC, Tannous E, Hailemariam S, Bose D, Makarova AV, Burgers PM, Zhang X. The Dimeric Architecture of Checkpoint Kinases Mec1ATR and Tel1ATM Reveal a Common Structural Organization. J Biol Chem. 2016 Jun 24;291(26):13436-47. doi: 10.1074/jbc.M115.708263. Epub 2016 Apr 28.
Stodola JL, Stith CM, Burgers PM. Proficient Replication of the Yeast Genome by a Viral DNA Polymerase. J Biol Chem. 2016 May 27;291(22):11698-705. doi: 10.1074/jbc.M116.728741. Epub 2016 Apr 12.

Kochenova OV, Bezalel-Buch R, Tran P, Makarova AV, Chabes A, Burgers PM, Shcherbakova PV. Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations. Nucleic Acids Res. 2016 Nov 29. pii: gkw1149. [Epub ahead of print]

Cho, J.-E., Huang, S.-Y.N., Burgers, P.M., Shuman, S., Pommier, Y., Jinks-Robertson, S. Parallel analysis of ribonucleotide-dependent deletions produced by yeast Top1 in vitro and in vivo. Nucleic Acids Res. 2016 Sep 19;44(16):7714-21. doi: 10.1093/nar/gkw495. Epub 2016 Jun 1.

Koc KN, Singh SP, Stodola JL, Burgers PM, Galletto R. Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ. Nucleic Acids Res. 2016 May 5;44(8):3811-9. doi: 10.1093/nar/gkw181. Epub 2016 Mar 21.

Santiago-Tirado FH, Onken MD, Cooper JA, Klein RS, Doering TL. Trojan Horse Transit Contributes to Blood-Brain Barrier Crossing of a Eukaryotic Pathogen. Bio. 2017 Jan 31;8(1). pii: e02183-16. Epub link

Wang X, Galletta BJ, Cooper JA, Carlsson AE. Actin-Regulator Feedback Interactions during Endocytosis. Biophys J. 2016 Mar 29;110(6):1430-43. doi: 10.1016/j.bpj.2016.02.018. PMID: 27028652. EPub link
Stark BC, Cooper JA. Differential expression of CARMIL-family genes during zebrafish development. Cytoskeleton (Hoboken). 2015 Oct;72(10):534-41. doi: 10.1002/cm.21257. Epub 2015 Oct 23.

Zhou JY, Szasz TP, Stewart-Hutchinson PJ, Sivapalan J, Todd EM, Deady LE, Cooper JA, Onken MD, Morley SC. L-Plastin promotes podosome longevity and supports macrophage motility. Mol Immunol. 2016 Oct;78:79-88. doi: 10.1016/j.molimm.2016.08.012. Epub 2016 Sep 7
Lanier MH, Kim T, Cooper JA. CARMIL2 is a novel molecular connection between vimentin and actin essential for cell migration and invadopodia formation. Mol Biol Cell. 2015 Dec 15;26(25):4577-88. doi: 10.1091/mbc.E15-08-0552. Epub 2015 Oct 14.

Xu, X., Wang, X., Todd, E.M., Jaeger, E.R., Vella, J.L., Mooren, O.L., Feng, Y., Hu, J., Cooper, J.A., Morley, S.C., Huang, Y.H. Mst1 kinase regulates the actin-bundling protein L-plastin to promote T cell migration. J Immunol. 2016 Sep 1;197(5):1683-91. doi: 10.4049/jimmunol.1600874. Epub 2016 Jul 27

Frieden C, Wang H, Ho CMW. A mechanism for lipid binding to apoE and the role of intrinsically disordered regions coupled to domain-domain interactions. Proc Natl Acad Sci U S A. 2017 May 30. pii: 201705080. doi: 10.1073/pnas.1705080114. [Epub ahead of print]

Mondal T, Wang H, DeKoster GT, Baban B, Gross ML, Frieden C. ApoE: In Vitro Studies of a Small Molecule Effector. Biochemistry. 2016 May 10;55(18):2613-21. doi: 10.1021/acs.biochem.6b00324. Epub 2016 Apr 27.
Wang H, Shu Q, Frieden C, Gross ML. Deamidation slows Curli Amyloid-Protein Aggregation. Biochemistry. 2017 May 26. doi: 10.1021/acs.biochem.7b00241. [Epub ahead of print]

Galburt EA, Tomko EJ. Conformational selection and induced fit as a useful framework for molecular motor mechanisms. Biophys Chem. 2017 Apr;223:11-16. doi: 10.1016/j.bpc.2017.01.004. [Epub 2017 Feb 3]

Garner AL, Rammohan J, Huynh JP, Onder LM, Chen J, Bae B, Jensen D, Weiss LA, Ruiz Manzano A, Darst SA, Campbell EA, Nickels BE, Galburt EA, Stallings CL. Effects of Increasing the Affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence. J Bacteriol. 2017 Jan 30;199(4). pii: e00698-16. doi: 10.1128/JB.00698-16. [Print 2017 Feb 15].
Galburt EA, Rammohan J. A Kinetic Signature for Parallel Pathways: Conformational Selection and Induced Fit. Links and Disconnects between Observed Relaxation Rates and Fractional Equilibrium Flux under Pseudo-First-Order Conditions. Biochemistry. 2016 Dec 20;55(50):7014-7022. [Epub 2016 Dec 8].

Rammohan, J., Ruiz Manzano, A., Garner, A.L., Prusa, J., Stallings, C.L., Galburt, E.A. Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD. Nucleic Acids Res. 2016 Sep 6;44(15):7304-13. doi: 10.1093/nar/gkw577. [Epub 2016 Jun 24]

Lisica A, Engel C, Jahnel M, Roldán É, Galburt EA, Cramer P, Grill SW. Mechanisms of backtrack recovery by RNA polymerases I and II. Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):2946-51. doi: 10.1073/pnas.1517011113. [Epub 2016 Feb 29].
Fishburn, J., Tomko, E., Galburt, E., Hahn, S. Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation. Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3961-6. doi: 10.1073/pnas.1417709112. [Epub 2015 Mar 16].

Sokoloski JE, Kozlov AG, Galletto R, Lohman TM. Chemo-mechanical pushing of proteins along single-stranded DNA. Proc Natl Acad Sci U S A. 2016 May 31;113(22):6194-9. doi: 10.1073/pnas.1602878113. [Epub 2016 May 16]
Singh SP, Koc KN, Stodola JL, Galletto R., A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-End. J Mol Biol. 2016 Mar 27;428(6):1053-67. doi: 10.1016/j.jmb.2016.02.017. [Epub 2016 Feb 22].

Greenberg MJ, Arpağ G, Tüzel E, Ostap EM. A Perspective on the Role of Myosins as Mechanosensors. Biophys J. 2016 Jun 21;110(12):2568-76. doi: 10.1016/j.bpj.2016.05.021. PMID: 27332116 [Epub link]

Greenberg, M.J., Shuman, H., Ostap, E.M., Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers. Methods in Molecular Biology. 2017, 1486 pp. 483 – 509, 2017. [Epub link]

Hayatshahi HS, Roe DR, Galindo-Murillo R, Hall KB, Cheatham TE. Computational Assessment of Potassium and Magnesium Ion Binding to a Buried Pocket in the GTPase-Associating Center RNA. J Phys Chem B. 2017 Jan 26;121(3):451-462. doi: 10.1021/acs.jpcb.6b08764. [Epub 2017 Jan 17].

Welty, R, Hall, KB. Nucleobases undergo dynamic rearrangements during RNA tertiary folding. J Mol Biol. 2016 Nov 6;428(22):4490-4502. doi: 10.1016/j.jmb.2016.09.015. [Epub 2016 Sep 29]

Chang, YK, Srivastava, Y, Hu, C, Joyce, A, Yang, X, Zuo, Z, Havranek, JJ, Stormo, GD, Jauch, R. Quantitative profiling of selective Sox/POU pairing on hundreds of sequences in parallel by Coop-seq. Nucleic Acids Res. 2017 Jan 25;45(2):832-845. doi: 10.1093/nar/gkw1198. [Epub 2016 Dec 2]

Zhang, C., Myers, C.A., Qi, Z., Mitra, R.D., Corbo, J.C., Havranek, J.J. Redesign of the monomer-monomer interface of Cre recombinase yields an obligate heterotetrameric complex. Nucleic Acids Res. 2015 Oct 15;43(18):9076-85. doi: 10.1093/nar/gkv901. [Epub 2015 Sep 13].

Liu G, Nash PJ, Johnson B, Pietzsch C, Ilagan MX, Bukreyev A, Basler CF, Bowlin TL, Moir DT, Leung DW, Amarasinghe GK. A Sensitive in Vitro High-Throughput Screen To Identify Pan-filoviral Replication Inhibitors Targeting the VP35-NP Interface. ACS Infect Dis. 2017 Mar 10;3(3):190-198. doi: 10.1021/acsinfecdis.6b00209. [Epub 2017 Feb 9]

Kraft TE, Heitmeier MR, Putanko M, Edwards RL, Ilagan MX, Payne MA, Autry JM, Thomas DD, Odom AR, Hruz PW. A novel FRET-based screen in high-throughput format to identify inhibitors of malarial and human glucose transporters. Antimicrob Agents Chemother.  2016 Nov 21;60(12):7407-7414. [Print 2016 Dec]

Thomas JJ, Abed M, Heuberger J, Novak R, Zohar Y, Beltran Lopez AP, Trausch-Azar JS, Ilagan MX, Benhamou D, Dittmar G, Kopan R, Birchmeier W, Schwartz AL, Orian A. RNF4-Dependent Oncogene Activation by Protein Stabilization. Cell Rep. 2016 Sep 20;16(12):3388-400. doi: 10.1016/j.celrep. 2016.08.024. [Epub link]

Spaulding CN, Klein RD, Ruer S, Kau AL, Schreiber HL, Cusumano ZT, Dodson KW, Pinkner JS, Fremont DH, Janetka JW, Remaut H, Gordon JI, Hultgren SJ. Selective depletion of uropathogenic E. coli from the gut by a FimH antagonist. 2017 June 14, Nature. Doi:10.1038 [Epub link]

Budelier MM, Cheng WW, Bergdoll L, Chen ZW, Janetka JW, Abramson J, Krishnan K, Mydock-McGrane L, Covey DF, Whitelegge JP, Evers AS. Photoaffinity labeling with cholesterol analogues precisely maps a cholesterol-binding site in voltage-dependent anion channel-1. J Biol Chem. 2017 Jun 2;292(22):9294-9304. doi: 10.1074/jbc.M116.773069. [Epub 2017 Apr 10]

Owusu BY, Thomas S, Venukadasula P, Han Z, Janetka JW, Galemmo Jr RA and Klampfer L. Targeting the tumor-promoting microenvironment in MET-amplified NSCLC cells with a novel inhibitor of pro-HGF activation. Oncotarget (2017) [Epud link]

Owusu BY, Galemmo R, Janetka J and Klampfer L. Hepatocyte Growth Factor, a Key Tumor-Promoting Factor in the Tumor Microenvironment. Cancers. 2017. 9(4), 35; doi:10.3390/cancers9040035 [Epub link]

Budelier MM, Cheng WW, Bergdoll L, Chen ZW, Abramson J, Krishnan K, Qian M, Covey DF, Janetka JW, Evers AS. Click Chemistry Reagent for Identification of Sites of Covalent Ligand Incorporation in Integral Membrane Proteins. Anal Chem. 2017 Feb 21;89(4):2636-2644. doi: 10.1021/acs.analchem.6b05003. [Epub 2017 Feb 9]  

Smith, J.A., Xu, G., Feng, R., Janetka, J.W., Moeller, K.D., C-Glycosides, Array-based Addressable Libraries, and the Versatility of Constant Current Electrochemistry.  Electroanalysis. 2016, 28 ( 11 ) pp. 2808 - 2817.                                
Wang Q, Heizer E, Rosa BA, Wildman SA, Janetka JW, Mitreva M. Characterization of parasite-specific indels and their proposed relevance for selective anthelminthic drug targeting. Infect Genet Evol. 2016 Apr;39:201-11. doi: 10.1016/j.meegid.2016.01.025. [Epub 2016 Jan 30]

Mydock-McGrane L.K., Hannan T.J., and Janetka J.W. Rational Design Strategies for FimH Antagonists: New Drugs on the Horizon for Urinary Tract Infection and Crohn’s Disease. Expert Opin Drug Discov. 2017 Jul;12(7):711-731. doi: 10.1080/17460441.2017.1331216. [Epub 2017 Jun 2]

Mydock-McGrane, L, Cusumano, Z, Han, Z, Binkley, J, Kostakioti, M, Hannan, T, Pinkner, JS, Klein, RD, Kalas, V, Crowley, J, Rath, NP, Hultgren, SJ, Janetka, JW. Anti-virulence C-mannoisdes as antibiotic-sparing, oral therapeutics for urinary tract infections. J Med Chem. 2016 Oct 27;59(20):9390-9408. [Epub 2016 Oct 14]
Jarvis C, Han Z, Kalas V, Klein R, Pinkner JS, Ford B, Binkley J, Cusumano CK, Cusumano Z, Mydock-McGrane L, Hultgren SJ, Janetka JW. Antivirulence Isoquinolone Mannosides: Optimization of the Biaryl Aglycone for FimH Lectin Binding Affinity and Efficacy in the Treatment of Chronic UTI. ChemMedChem. 2016 Feb 17;11(4):367-73. doi: 10.1002/cmdc.201600006. [Epub 2016 Jan 26]

Kinch M.S. and Woodard P.K. Analysis of FDA-approved imaging agents. Drug Discov Today. (2017) [Epub link]

Krishna, U., Romero-Gallo, J., Suarez, G., Azah, A., Krezel, A.M., Varga, M.G., Forsyth, M.H., Peek, R.M. Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease. J Infect Dis. 2016 Aug 15;214(4):644-8. doi: 10.1093/infdis/jiw189. [Epub 2016 May 10]

Simon MJ, Sokoloski JE, Hao L, Weiland E, Lohman TM. Processive DNA Unwinding by RecBCD Helicase in the Absence of Canonical Motor Translocation. J Mol Biol. 2016 Jul 31;428(15):2997-3012. doi: 10.1016/j.jmb.2016.07.002. Epub 2016 Jul 14. PMID: 27422010  [Epud link]

Ballante F, Reddy DR, Zhou NJ, Marshall GR. Structural insights of SmKDAC8 inhibitors: Targeting Schistosoma epigenetics through a combined structure-based 3D QSAR, in vitro and synthesis strategy. Bioorg Med Chem. 2017 Apr 1;25(7):2105-2132. doi: 10.1016/j.bmc.2017.02.020. [Epub 2017 Feb 13]

Reddy DR, Ballante F, Zhou NJ, Marshall GR. Design and synthesis of benzodiazepine analogs as isoform-selective human lysine deacetylase inhibitors. Eur J Med Chem. 2017 Feb 15;127:531-553. doi: 10.1016/j.ejmech.2016.12.032. [Epub 2016 Dec 18]
Istvan ES, Mallari JP, Corey VC, Dharia NV, Marshall GR, Winzeler EA, Goldberg DE. Esterase mutation is a mechanism of resistance to antimalarial compounds. Nat Commun. 2017 Jan 20;8:14240. [Epub link]

Ballante F, Marshall GR. An Automated Strategy for Binding-Pose Selection and Docking Assessment in Structure-Based Drug Design. J Chem Inf Model. 2016 Jan 25;56(1):54-72. doi: 10.1021/acs.jcim.5b00603. [Epub 2016 Jan 6.]

Needham SR, Roberts SK, Arkhipov A, Mysore VP, Tynan CJ, Zanetti-Domingues LC, Kim ET, Losasso V, Korovesis D, Hirsch M, Rolfe DJ, Clarke DT, Winn MD, Lajevardipour A, Clayton AH, Pike LJ, Perani M, Parker PJ, Shan Y, Shaw DE, Martin-Fernandez ML. EGFR oligomerization organizes kinase-active dimers into competent signalling platforms. Nat Commun. 2016 Oct 31;7:13307. [Epub link]

Ronan T, Macdonald-Obermann JL, Huelsmann L, Bessman NJ, Naegle KM, Pike LJ. Different Epidermal Growth Factor Receptor (EGFR) Agonists Produce Unique Signatures for the Recruitment of Downstream Signaling Proteins
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Liu T, Dai A, Cao Y, Zhang R, Dong MQ, Wang HW. Structural Insights of WHAMM's Interaction with Microtubules by Cryo-EM. J Mol Biol. 2017 May 5;429(9):1352-1363. doi: 10.1016/j.jmb.2017.03.022. [Epub 2017 Mar 25]


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